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Fig. 4. Light microscopy analysis of PV formation. Macrophages treated with IFN-{gamma} were infected with L. amazonensis metacyclic promastigotes (A-D) or amastigotes (E-H). At the time points indicated, macrophages were fixed and then examined by phase-contrast microscopy. In each panel, some parasites are indicated by white arrowheads. Bar, 10 µm.