Fig. 12. Confocal microscopy analysis of the association of late endosome/lysosome `markers' with early phagosomes formed after internalization of L. amazonensis amastigotes. IFN--pre-treated macrophages were infected (four parasites/host cell) and 30 minutes later processed for immunofluorescence microscopy. Cell preparations were incubated with immune sera or Abs directed against rab7p (A), lamp-1 (B) or cathepsin B (C) and then with adequate fluorescein conjugates (green staining). In B and C, cells were also stained with propidium iodide to visualize macrophage nuclei and parasite nuclei and kinetoplasts (red staining). Optical sections (0.3-0.5 µm thickness) are shown. The micrographs are representative of two separate experiments. Bars, 2 µm.