Fig. 11. Kinetics of endocytic `marker' association with phagosomes formed after internalization of L. amazonensis amastigotes. IFN--treated macrophages were infected at a multiplicity of four parasites/host cell. At various times post-infection, they were processed as described in Fig. 5 and the association of the following molecules with parasite-containing compartments was examined: transferrin receptor, EEA1, rab7p, macrosialin, lamp-1, cathepsin B and MHC class II molecules. For each experiment and at each time point, the percentages of parasite-containing organelles displaying the molecules listed above were determined after counting about 100 organelles. Data represent the means + or - range of two separate experiments.