Fig. 1. Hck vesicles belong to the endocytic pathway. Adherent MDMs were incubated with the endocytic tracer, rhodamine-dextran (Rho-dextran). After a chase of five hours, cells were fixed and labeled with a monoclonal anti-CD63 Ab or polyclonal anti-Hck Abs revealed by FITC-conjugated Abs. Colocalization of Rho-dextran with CD63 (compare B with A) or Hck (compare E with D) appears in yellow in the merged picture (C and F). Data are from one experiment that is representative of four independent experiments. Bar, 7 µm.