Fig. 5. NIH3T3 cell in late prometaphase co-stained for m53BP1 (red) and ACA (green) showed a stronger 53BP1 signal on the kinetochores of unaligned chromosomes (indicated with arrows) than those on aligned chromosomes (a,b,c), whereas the level of ACA signal was more homogeneous on all kinetochores (b,d). DNA stained with DAPI is shown in blue (a). Images were recorded with a Delta Vision microscope.