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Fig. 1. Western blotting showing the specificity of two anti-m53BP1 antibodies. Extracts prepared from mouse NIH3T3 cells were probed with affinity-purified antibodies (I) against N-m53BP1 (lane 2) and those against C-m53BP1 (lane 4). Both antibodies specifically recognised a single protein band migrating at about 300 kDa. The pre-immune sera (P) from the same rabbits gave no or very little signal (lanes 1,3).