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Fig. 5. LAMP-1 distribution in cells expressing EGFP-rab5a wt or EGFP-rab5aS34N. EcR293pIND-Hygro /EGFP-rab5a wt cells (A) and EcR293pIND-Hygro/EGFP-rab5a S34N cells (B) were induced for 72 hours with 5 µM ponasterone, then fixed and labeled with anti-LAMP-1 primary and TRITC (red) secondary antibodies. The labeled cells were imaged by deconvolution fluorescence microscopy. Bar, 10 µm.