Fig. 3. Lysosome/late endosome proteins localize with EGFP-rab5aQ79L. EcR293 pIND-Hygro/EGFP-rab5aQ79L cells were treated with vehicle (left) or 5 µM ponasterone (right) for 72 hours and then fixed and labeled with antibodies against LAMP-1, LAMP-2, cathepsin D or CI-M6PR as indicated. Secondary antibodies were TRITC (red) goat anti-mouse or goat anti-rabbit IgG. The labeled cells were imaged by deconvolution fluorescence microscopy. Bar, 10 µm.