Fig. 2. Rapid labeling of EGFP-rab5a-containing vesicles with transferrin Texas Red. EcR293 pIND-Hygro/EGFP-rab5a wt cells (A) and EcR293 pIND-Hygro/EGFP-rab5aQ79L cells (B) were grown for 72 hours in the presence of 5 µM ponasterone, then incubated for 5 minutes with transferrin Texas Red (20 µg/ml) prior to rapid fixation in 4% paraformaldehyde. The cells were imaged by deconvolution fluorescence microscopy. Bar, 10 µm.