Fig. 6. Some lamin A mutations increase the loss of emerin from the nuclear envelope in transfected cells. (A) Laser scanning confocal immunofluorescence microscopy images of C2C12 cells transfected with wild-type (WT) or indicated mutated prelamin A, fused to a FLAG-epitope. In cells expressing mutants causing an increase in emerin loss from the nuclear envelope, examples are shown both of cells with correctly localized and mislocalized emerin. Antibodies used were anti-FLAG monoclonal antibodies recognized by FITC-conjugated secondary antibodies (left) and polyclonal antibodies against emerin recognized by rhodamine-conjugated secondary antibodies (middle). The panels to the right show an overlay of the FITC (green) and rhodamine (red) channels. Bars, 10 µm. (B) Percentage of cells with lack of emerin in the nuclear envelope in cells expressing wild-type (WT) or mutant lamin A. One hundred cells each in two separate experiments were studied. P values denote a statistically significant difference between the mutant and wild-type as determined by ²-test. NS denotes no significant difference.