Fig. 5. Endogenous lamin A/C is localized both to the nuclear foci and to the nuclear rim. Staining with monoclonal antibodies against FLAG, recognizing exogenous protein, and polyclonal anti-lamin A/C antibodies, recognizing both exogenous and endogenous A-type lamins. The monoclonal antibodies were recognized by FITC-conjugated secondary antibodies and the polyclonal antibodies were recognized by rhodamine-conjugated secondary antibodies. The panels show an overlay of the FITC (green) and rhodamine (red) channels. Areas of colocalization appear yellow. Arrows show lamin A/C localized to the nuclear envelope. Bars, 10 µm.