Fig. 4. Nuclear pore complexes are not present in nuclear foci. The panels show laser scanning confocal immunofluorescence microscopy images of C2C12 cells transfected with prelamin A with missense mutations as indicated. Short arrows indicate lack of pore complexes in the nuclear foci and long arrows their absence from one pole of some nuclei. Antibodies used were monoclonal antibodies against nuclear pore complex protein p62 (NPC) (left panel) and polyclonal antibodies against lamin A/C (middle panel). The monoclonal antibodies were recognized by FITC-conjugated secondary antibodies and the polyclonal antibodies were recognized by rhodamine-conjugated secondary antibodies. The panels to the right show an overlay of the FITC (green) and rhodamine (red) channels. Bars, 10 µm.