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Fig. 3. Analysis of lamin B2 and LAP2 localization in cells expressing lamin A mutants. The panels show laser scanning confocal immunofluorescence microscopy images of C2C12 cells transfected with the prelamin A E358K or {Delta}NLA mutants fused to a FLAG-epitope. (A) Staining with monoclonal antibodies against lamin B2 (left panel) and polyclonal antibodies against lamin A/C (middle panel). Arrows indicate concentration of lamin B2 by nuclear foci and absence of lamin B2 from one pole of some nuclei. (B) Staining with monoclonal antibodies against FLAG (left panel) and polyclonal antibodies against LAP2 (middle panel). The monoclonal antibodies were recognized by FITC-conjugated secondary antibodies and the polyclonal antibodies were recognized by rhodamine-conjugated secondary antibodies. The panels to the right show an overlay of the FITC (green) and rhodamine (red) channels. Bars, 10 µm.