Fig. 8. Loss of tension results in Bub1 recruitment to kinetochores. Projections of deconvolved image stacks showing metaphase HeLa cells stained with Hoechst (blue, top panels), 4B12 (anti-Bub1) and anti-centromere antibodies (red and green respectively in the merged image shown in the middle and bottom panels). The bottom panels show enlarged views of the boxed areas shown in the middle panels. Scale bars represent 5 µm. (A) In the absence of taxol, the metaphase is broad, centromeres are stretched and Bub1 staining at kinetochores is weak. (B) Following 30 minutes of taxol treatment, the metaphase is tight, centromere stretching is reduced and Bub1 staining at kinetochores is increased.