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Fig. 4. Monoclonal and polyclonal anti-Bub1 antibodies give identical patterns. A deconvolved image of a prometaphase DLD-1 cell stained with (A) 4B12 and (B) SB1.3 anti-Bub1 antibodies. (C) shows a red/green merged image and (D) shows the Hoechst-stained chromosomes. The scale bar represents 5 µm. Both 4B12 and SB1.3 give identical patterns.