Fig. 2. Bub1 and BubR1 localise to the same domain within the kinetochore. (A) Projection of a deconvoluted image stack showing DLD-1 prometaphase kinetochore pairs stained with 4B12 (anti-Bub1, red) and RCE1 (anti-Cenp-E, green), showing that localisation of Cenp-E extends distally beyond Bub1. (B) 3D model showing that Cenp-E also extends beyond Bub1 in the z axis. (C) Projection of a deconvolved image stack showing a nocodazole-treated DLD-1 cell stained with 4B12 (anti-Bub1, red) and SBR1.1 (anti-BubR1, green). Because Bub1 and BubR1 colocalise perfectly the kinetochores appear orange in this merged image. Scale bar represents 5 µm. (D) 3D model of one of the kinetochores from (C) showing perfect overlap of Bub1 and BubR1.