JCS -- Trinkle-Mulcahy et al. 114 (23): 4219 Figure IG2

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Fig. 2. Localization of FP-tagged PP1 isoforms in transiently transfected HeLa cells. Panel A shows the localization of EYFP-PP1 ${alpha}$ in live HeLa cells, with an accompanying DIC (differential interference contrast) image in Panel B and the two images merged in Panel C. EGFP-PP1ß/ ${delta}$ localization is shown the same way in Panels D-F, and EYFP-PP1 ${gamma}$ localization in Panels G-I. PP1 ${alpha}$ and PP1 ${gamma}$ maintain their specific localization patterns when co-expressed in the same cells as ECFP-PP1 ${alpha}$ (Panel J) and EYFP-PP1 ${gamma}$ (Panel K). Panel L shows the two patterns merged, with ECFP-PP1 ${alpha}$ pseudocolored green and EYFP-PP1 ${gamma}$ pseudocolored red. Arrowheads indicate nucleoli. Scale bars are 10 µM.