Fig. 2. Mad2 antibodies recognize a protein of approximately 24 kDa on an immunoblot of grasshopper testes. Coomassie blue-stained SDS-PAGE of molecular weight markers (MW), bacterially expressed Xenopus Mad2 protein (XMad2), and homogenized grasshopper testes (GH) is shown on the left. A blot of homogenized grasshopper testes labeled with Mad2 antibodies is shown on the right. The blot lane was run on the same gel shown stained on the left (see Materials and Methods).