Fig. 6. CD95- and TRAIL-mediated apoptosis in Jurkat cells overexpressing Bcl-2 at different intracellular locations. Cells were stimulated with 100 ng/ml of either anti-CD95 (A) or TRAIL (B). After the indicated times apoptosis was quantified by flow cytometry. The experiments reveal that overexpression of the Bcl-2 mutants had no significant effect on death receptor-mediated apoptosis. The error bars indicate the standard deviations from independent measurements of the same cell batch (five independent measurements).