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Fig. 5. Loss of mitochondrial membrane potential after irradiation. Cells were irradiated with 10 Gy and the mitochondrial membrane potential {Delta}{Psi}m was determined by flow cytometry. Bcl-2 overexpression at mitochondria (Bcl-2/MT), the ER (Bcl-2/ER) or in both compartments (Bcl-2/WT) protected cells from radiation-induced mitochondrial potential breakdown, whereas Bcl-2/{Delta}TM had no significant effects. The error bars indicate the standard deviations from independent measurements of the same cell batch.