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Fig. 2. Induction of apoptosis by ionizing irradiation. Immunoblot analysis with an anti-Bcl-2 antibody revealed that the transfectants expressed the different forms of Bcl-2 at roughly similar levels (A). Apoptosis induction in control or cells irradiated with 10 Gy was quantified by flow cytometry (B). Overexpression of Bcl-2/MT and Bcl-2/WT strongly protected cells against apoptosis. Similar to Bcl-2/MT, ER-targeted Bcl-2 conferred survival against irradiation-induced cell death. Cells expressing the cytosolic, non-membrane Bcl-2/{Delta}TM mutant revealed apoptosis to similar levels as vector control cells. The error bars indicate the standard deviations from independent measurements of the same cell batch (five independent experiments).