Fig. 5. Internalization of IL-2R is blocked by AlF₃. Kit225 K6 cells were labeled with anti-Tac fab against the IL-2R. Cells were incubated in the absence (control sample, ) or in the presence () of AlF₃ at 37°C. Samples were taken every 20 minutes, and the ratio of intracellular fluorescence was determined with the acid-wash method as described in Materials and Methods. Results are mean±s.e.m.