Fig. 8. Cx46 transport is inhibited by Cx43/ß-gal. Control (con; A-C), 43ß1 (D-F) and 43ß2 (G-I) cells were transiently transfected with Cx46 and allowed to recover for 48 hours. The cells were fixed, permeabilized and then double-label immunostained using mouse anti-ß-gal (A,D,G) and rabbit anti-Cx46 (B,E,H) antisera, which were detected using Texas Red-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG as secondary antibodies, respectively. Cells showed colocalization of Cx43/ß-gal with both Cx46 at the cell surface (arrows) and Cx46 retained in the perinuclear region of the cell (arrowheads). Note the increased retention of Cx46 by 43ß2 cells, as compared to 43ß1 cells. Bar, 10 µm.