Fig. 2. Cx43/ß-gal expression alters the intracellular distribution of endogenous Cx43. Control (A-C), 43ß1 (D-F) and 43ß2 (G-I) cells were fixed, permeabilized and then double-label immunostained using mouse anti-ß-gal (A,D,G) and rabbit anti-Cx43 (B,E,H), detected using Texas Red-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG as secondary antibodies. With increasing Cx43/ß-gal expression, there was increased perinuclear localization of both the anti-ß-gal and anti-Cx43 signals (arrowheads). Also, while control cells had numerous areas showing labeling by Cx43 alone in areas where cells are in close apposition (arrows), this was less apparent for cells expressing high levels of Cx43/ß-gal. Note the plasma membrane regions of 43ß1 cells, which showed labeling by both anti-ß-gal and anti-Cx43 (open arrows). Bar, 10 µm.