Fig. 6. Effect of wortmannin on the subcellular localisation of GFP-FENS-1, GFP-iFYVE FENS-1 and GFP-DFCP1. COS-7 cells were transfected with expression vectors encoding the indicated GFP-tagged proteins and processed for live image recording of GFP-fluorescence as described in the Materials and Methods. The images shown represent stills taken at the indicated times after 100 nM wortmannin addition: the original videos describing the effects of wortmannin on the localisation of GFP-FENS-1 (Movie A) and GFP-iFYVE FENS-1 (Movie C) can be viewed at jcs.biologists.org/supplemental The cytosolic localisation of GFP-[C347S]FENS-1 is shown as an inset in the top left-hand panel.