Fig. 5. Colocalisation of GFP-FENS-1 and GFP-iFYVE FENS-1 (the isolated FYVE domain) with the early endosomal marker EEA1. COS-7 cells were transfected with expression vectors encoding GFP-FENS-1 or GFP-iFYVE FENS-1. The cells were fixed with paraformaldehyde and processed for direct (GFP) and indirect immunofluorescence (Texas Red, anti-endogenous EEA1) as described in the Materials and Methods.