Fig. 4. Subcellular localisation of exogenously expressed FENS-1 and DFCP1. Top two panels: COS-7 cells were transfected with expression vectors encoding GFP-FENS-1 and GFP-DFCP1 and processed for live image recording of GFP-fluorescence as described in the Materials and Methods. The images shown represent stills taken from Movies A and B describing GFP-FENS-1 and GFP-DFCP1, respectively: the movies can be viewed at jcs.biologists.org/supplemental. Lower panel: COS-7 cells were co-transfected with expression vectors encoding GFP-FENS-1 and Myc-DFCP1. The cells were fixed with methanol and processed for direct (GFP) and indirect (Texas-Red, anti-Myc) fluorescence as described in the Materials and Methods.