Fig. 6. Mislocalization of prolactin in cells expressing mutant GH. Cells cotransfected with wt-GH or 32-71-GH and human prolactin were stained with antibody to prolactin (left panels) and antibody to GH (middle panels). Right panels show GH in red and prolactin in green; colocalized staining appears yellow. Prolactin is found in the Golgi apparatus of cells expressing wt-GH but retained in the ER in cells expressing 32-71-GH. PRL, prolactin.