Fig. 3. Golgi marker and GH staining. Cells cotransfected with wt-GH or 32-71-GH were stained with antibody to the Golgi markers ß-COP (top left panels), Golgi 58K (middle left panels), or membrin (bottom left panels), and antibody to GH (center column of panels). Merged images show GH in red and Golgi markers in green; colocalized staining appears yellow. Where noted, 10 µg/ml tunicamycin was added for 3 hours during transfection and then removed. Arrowheads denote cells in which the Golgi almost disappeared and arrows show cells in which the Golgi was visible but dispersed.