Fig. 1. Western blots for GH and ß-COP. Cells were transfected with wt-GH, 32-71-GH or P89L-GH as shown at the top of gels. As shown on the left, wt-, P89L- and 32-71-GH were expressed at similar levels. The predicted size for wt- and P89L-GH is 22 kDa and for 32-71-GH is 17.5 kDa. As shown on the right, transfection of cells with wt-GH or 32-71-GH does not affect expression of endogenous ß-COP, so the disappearance of ß-COP staining in cells expressing 32-71-GH was not due to decreased ß-COP expression.