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Fig. 3. Stimulation of GRC cell-surface expression by HA and inhibition of translocation by the Ca2+-channel blocker SK&F 96365. BON cells were incubated for 0, 10 and 20 minutes with 2 nM HA, then for 20 minutes at 37°C with the antiserum against GRC (top panel). GRC translocation was blocked if NH15-CA2 cells were pretreated for 20 minutes with SK&F 96365 (10 µM), before HA (2 nM) was added (bottom panel).