Fig. 8. The effect of bicarbonate and albumin on cholesterol and phospholipid scrambling in incubated boar sperm samples. Ejaculates from six different boars were incubated in HBT, HBT supplemented with 0.3% (w/v) albumin (HBT-BSA), for 2 hours. Cholesterol was fluorescently visualized by filipin and phospholipid scrambling by M540 (see Materials and Methods). Sperm cells showing the filipin pattern depicted in Fig. 7C (pattern C) were scored as cells featuring cholesterol efflux (filled bar), whereas filipin pattern B (Fig. 7B) were scored as cells featuring cholesterol redistribution (hatched bar). Sperm cells showing high M540 fluorescence (Fig. 2f) were scored as cells that underwent phospholipid scrambling (open bar). After each incubation condition, 200 cells were counted in triplicate, the resulting mean values were calculated from all six boars. From these data mean values with s.d. are expressed (n=6).