Fig. 9. Assembly of -SMA induced by P1F6 in HFL-1 cells is disrupted by agents that inhibit collagen gel contraction. HFL-1 cells were incubated with or P1F6 in the presence of PKC inhibitor calphostin c (a), tyrosine kinase inhibitor genestein (b), src inhibitor pp2 (c), MAPK inhibitor PD98059 (d), PI-3 kinase inhibitor wortmannin (e) or Rho-GTPases inhibitor C3-exoenzyme (f). Dual-label images of HFL-1 cells were obtained by confocal immunofluoresence microscopy of fixed cells following staining with -SMA (green) and TRITC-phalloidin to label F-actin (red). Bar, 50 µm.