JCS -- Scaffidi et al. 114 (19): 3507 Figure IG8

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Fig. 8. Contraction of FPCL is induced following exposure of HFL-1 to function-blocking antibodies against VN integrins. (A) Fibroblast populated collagen gels were exposed to culture medium alone ( ${blacksquare}$ ), P1F6 ( ${blacktriangleup}$ ), MAPK inhibitor PD98059 ( ${blacktriangledown}$ ) or P1F6 in the presence of PD98059 ( ${blacklozenge}$ ), PKC inhibitor calphostin c, PI-3 kinase inhibitor wortmannin, Rho-GTPases inhibitor C3-exoenzyme, F-actin inhibitor cytochalasin D or tyrosine kinase inhibitor genestein (all ). Gel dimensions were determined after 24, 48 and 72 hours. (B) Fibroblast populated collagen gels were exposed to culture medium alone ( ${blacksquare}$ ) or VN 1 µg/ml ( ${blacktriangleup}$ ) or 10 µg/ml ( ${blacktriangledown}$ ). Gel dimensions were determined after 24, 48 and 72 hours. Results are calculated from duplicate wells of at least four different experiments and are expressed as mean±s.e.m. *Significantly less gel area compared to cells exposed to medium alone, P<0.05.