Fig. 7. Assembly of -SMA and F-actin in HFL-1 cells is induced by function-blocking antibodies to VN integrins. HFL-1 cells were incubated with no antibody (a) or LM609 (anti-vß3, b), P5D2 (anti-ß1, c), P1F6 (anti-vß5, d), 10D5.8 (anti-ß6, e) or with VN itself (f). Dual-label images of HFL-1 cells were obtained by confocal immunofluoresence microscopy of fixed cells following staining with -SMA (green) and TRITC-phalloidin to label filamentous actin (red). Yellow/orange staining represents intense areas of colocalization of green and red fluorescence. Bar, 50 µm.