Fig. 6. Cells were treated with the translation inhibitor cyclohexamide (10 µM) or the transcription inhibitor actinomycin D (1 µM) for 45 minutes prior to incubation with P1F6 (A) LM609 (B) or P5D2 (C) for a further 24 hours. Expression of -SMA was analyzed using ELISA. Results are calculated from triplicate wells of at least four different experiments and are expressed as mean±s.e.m. *Significantly less -SMA expression compared to cells exposed to function-blocking antibody alone, P<0.05.