Fig. 2. Detection of VN on HFL-1 cells. HFL-1 cells were stained with a monoclonal antibody recognizing VN followed by FITC-labeled goat anti-mouse IgG. Sections were counterstained with DAPI to visualize cell nuclei (blue) and analyzed using confocal microscopy. (a) A pseudo coloured z-series projection shows specific VN immunoreactivity (green stain) in the intracellular compartment and on the cell membrane (arrows). DAPI staining of nuclei is shown in blue. (b) A higher magnification single optical section shows intense localization to the cell membrane (arrowhead). Bar, 100 µm.