Fig. 1. Integrins recognizing VN are present on HFL-1 cells. (A) HFL-1 cells were stained with no antibody (white peaks) or with monoclonal antibodies LM609 (anti-vß3), P1F6 (anti-vß5), P5D2 (anti-ß1) or L230 (anti-v) (black peaks) followed by FITC-labeled rabbit anti-mouse IgG. Immunostaining was then analyzed by flow cytometry. (B) In lane 1, cells were surface biotinylated, lysed and cell extracts immunoprecipitated with L230 (anti v). In lane 2, untreated cells were lysed and extracts were immunoprecipitated with L230. P5D2 (anti ß1) was used in western analysis to establish that the vß1 complex was present in HFl-1 cells.