Fig. 7. Colocalization of PKA RII subunits with integrins expressing the 12G10 epitope following integrin activation. HT-1080 cells were treated with 10 µM Sp-cAMPS for 20 minutes, and plated on poly-D-lysine coated cover slips for another 20 minutes in the presence of no antibody or 10 µg/ml of 12G10 directly labeled with Alexa^TM 568. PKA RII subunits were visualized by indirect immunofluorescence using fluorescently labeled donkey anti-goat IgG antibody (green) and ß₁ integrins were visualized with Alexa^TM 568-labeled 12G10 (red). Fluorescent images were combined to determine colocalization (yellow). All bars, 10 µm.