Fig. 2. Inhibition of cell attachment to collagen type IV with inhibitors of PKA and PKC. HT-1080 fibrosarcoma cells were assayed for cell adhesion to 0.2 µg/ml collagen type IV in the presence of no inhibitor (white bars), 20 µM MPKI peptide (grey bars) or 2 µM calphostin C (black bars). Cells were incubated with 10 µg/ml anti-ß₁ integrin antibodies and allowed to attach to substrate for 20 minutes. The amount of nonspecific attachment, determined using wells coated with BSA alone (<5%), was subtracted from each point. Values shown are mean±s.d. (n=4).