Fig. 1. MAb 12G10-induced cell-cell adhesion is dependent on PKA. The effects of (A) 100 nM staurosporine, 2 µM calphostin C and 150 µM genistein, (B) H89 and (C and F) MPKI peptide on HT-1080 cell-cell adhesion in the presence of 10 µg/ml 12G10. (D) HT-1080 cell-cell adhesion with no treatment, (E) 10 µg/ml 12G10 and (F) cells treated with 20 µM MPKI peptide prior to the addition of 10 µg/ml 12G10. CCAI was scored and defined as described in Materials and Methods. In (A), the data are expressed relative to the level of HT-1080 cell-cell adhesion with mAb 12G10 (100%). The dotted lines (A), (B) and (C) represent the level of basal HT-1080 cell-cell adhesion±s.d. Values shown are mean±s.d. (n=8). Bar, D-F, 100 µm.