Fig. 2. Different locations of wild-type and mutated RII-GFP in mitotic cells. Reh cells were transiently transfected with GFP (A,B), RII-GFP (C,D), RII(T54E)-GFP (E,F), RII(T54L)-GFP (G,H) or RII(T54V)-GFP (I,J), fixed and stained with affinity-purified anti-AKAP450 polyclonal antibodies to stain centrosomes (I,J) and with Hoechst 33342 to stain DNA (inserts). GFP fluorescence was examined in interphase (A,C,E,G,I) and mitotic (B,D,F,H,J) cells. Arrows indicate the centrosomal association of RII.