Fig. 9. Both DdRab11 and RabD no longer associates with the proton pump following treatment of cells with the ATPase inhibitor concanamycin A. Cells growing in HL5 medium we exposed to concanamycin A (2 µM final concentration) for 1 hour, then fixed and prepared for fluorescence microscopy using antibodies to DdRab11 (A), RabD (D) and the 100 kDa proton pump subunit (B,E). Arrows indicate proton pump positive vacuoles that show little staining for DdRab11 or RabD. The cell stained for DdRab11 (A) and 100 kDa proton pump subunit (B) was also photographed under bright field conditions (C). Scale bar: 5 µm.