Fig. 8. DdRab11 no longer co-localizes with RabD or the proton pump in cells exposed to hypo-osmotic conditions. Cells attached to coverslips were placed in distilled water for 20 minutes. Cells were fixed and decorated with antibodies to RabD (A), DdRab11 (C) and the proton pump subunit (B,D). Cells were viewed in a fluorescence microscope. White arrows (A,B) show co-localization of RabD and the proton pump. Arrowheads (C,D) show DdRab11 and the proton pump, respectively, not co-localized. The arrow in D indicates a swollen vesicle in a cell stained for the proton pump. The bright field image of this same cell is shown in E. Scale bar: 5 µm.