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Fig. 5. GFP-DdRab11 associates with expulsing contractile vacuoles. Images 1-12 represent a photographic sequence from a 12 second movie of unfixed cells expressing GFP-DdRab11. The sponguimal network and bladders are easily visualized by the GFP-tagged DdRab11. The CV at the six o'clock position is shown in the process of water expulsion.