Fig. 4. GFP-DdRab11 co-localizes with the 100 kDa proton pump subunit in the contractile vacuole. Cells expressing CV-localized GFP-DdRab11 were observed and photographed (A,B). Co-localization experiments in cells expressing GFP-DdRab11 are shown in C-E. (C) GFP-DdRab11; (D) Texas Red-tagged antibody staining of the 100 kDa ATPase proton pump protein. (E) A dual fluorescent image of both C,D that demonstrates complete co-localization of GFP-DdRab11 and the 100 kDa ATPase proton pump protein. Scale bar: 5 µm.