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Fig. 3. DdRab11 co-localizes with the 100-kDa vacuolar ATPase in the contractile vacuole. Indirect immunofluorescence techniques were used to stain and visualize fixed cells for DdRab11 (A), ATPase (B,D) and RabD (C). Arrows denote reticular elements that contain DdRab11, RabD, and the 100 kDa proton pump subunit. Scale bars: 5 µm.