Fig. 12. Cells expressing DdRab11N125I are normal in endocytic uptake and release rates but are enhanced in the rate of phagocytosis. DdRab11N125I (+) cells were incubated in HL5 medium containing FITC-dextran (1 mg/ml). At the indicated times (top), cells were recovered and washed by centrifugation and accumulated FITC-dextran was measured using a spectrophotometer. In addition (middle), after 3 hours of accumulation, cells were washed and resuspended in growth medium. At the indicated times, cells were washed and the FITC-dextran remaining intracellular was measured. Finally (bottom), cells in growth medium were incubated with fluorescent latex beads (at the indicated times), the cells were washed, and accumulated beads were measured using a spectrophotometer.