Fig. 10. DdRab11N125I-expressing cells are defective in contractile vacuolar function. Cells expressing a DdRab11N125I dominant negative protein were generated and contractile vacuolar function was tested. Wild-type (A) and mutant cells (B) grown in growth media were placed in distilled water and photographed. Photomicrographs of wild-type cells in water (A) show cells with amoeboid morphology and slightly enlarged contractile vacuoles (black arrowhead). However, mutant cells in water (B) were detached from the plastic and contained rounded spherical shapes with distinct enlarged contractile vacuoles (black arrowheads).