
Fig. 3 Analysis of mog1 in Schizosaccharomyces pombe. (A) Tetrad
analysis of FJN1, a strain in which one genomic copy of mog1 is
replaced with a KanR cassette. Two out of the four haploid
spores in each column germinated, indicating a requirement of mog1
for viability. (B) Complementation analysis in S. pombe. Growth of
colonies derived from germinated spores carrying
mog1
transformed with plasmids encoding S. pombe Mog1p (SpMog1), S.
cerevisiae Mog1p (ScMog1) or Xenopus laevis Mog1 (XMog1) under
the control of the thiamine-repressible nmt1 promoter.